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EMSCAN Corporation
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Molecular Devices LLC
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Lincoln Electric
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Bio-Rad
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OHAUS Corporation
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Fondriest Environmental
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Shanghai INESA Scientific
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Jenway Inc
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Sper Scientific
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METTLER TOLEDO
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Advanced Energy Industries Inc
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ADInstruments
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Image Search Results
Journal: Chembiochem
Article Title: Modulation and Visualization of EF‐G Power Stroke During Ribosomal Translocation
doi: 10.1002/cbic.201900276
Figure Lengend Snippet: Design, purification, and activity assay of crosslinked EF‐Gs. A) Crosslinking residues F411 and Y535 (bacterial numbering). B) Separation by continuous elution electrophoresis. Lanes 1 and 4: pure lower and upper bands, respectively; lanes 2 and 3: the transitions from one component to the other. Lanes are numbered from left to right. C) Purification by MiniGel and hand‐incision, followed by Bio‐Rad Model 422 elution. Lanes 1 and 3: purified CL EF‐G with different quantities; lane 2: mixture of un‐crosslinked and crosslinked; lane 4: purified un‐crosslinked EF‐G; lane 5: marker. D) Purification by activated thiol Sepharose 4B. Lanes 1–3 were analyzed after 1 h, 4 h, and overnight incubation time with the beads, respectively. The final purity was approximately 70 %. E) Purification by maleimide‐activated magnetic beads. Lanes 1–3 were analyzed after 1, 2, and 3 h of incubation time, respectively. The final purity was >90 %. F) Radioactivity measurements by the poly(Phe) assay for the WT and two crosslinked EF‐Gs.
Article Snippet: The electroelution was conducted with the
Techniques: Purification, Activity Assay, Electrophoresis, Marker, Incubation, Magnetic Beads, Radioactivity